Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by a widely-used RT-PCR assay
Sabrina A Mann,
James T Webber,
Sidney M. Bell,
Si Noon Hong,
Minh Phuong Nguyen,
Lienna Y Chan,
Karan D Bhatt,
Angela M. Detweiler,
Debra A Wadford,
Norma F. Neff,
Cristina M Tato,
Joseph L. Derisi,
Posted 26 Aug 2020
bioRxiv DOI: 10.1101/2020.08.25.265074
Posted 26 Aug 2020
We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets for robust SARS-CoV-2 detection. ### Competing Interest Statement The authors have declared no competing interest.
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