Identification of a polymorphism in the N gene of SARS-CoV-2 that adversely impacts detection by a widely-used RT-PCR assay
By
Manu Vanaerschot,
Sabrina A Mann,
James T Webber,
Jack Kamm,
Sidney M. Bell,
John Bell,
Si Noon Hong,
Minh Phuong Nguyen,
Lienna Y Chan,
Karan D Bhatt,
Michelle Tan,
Angela M. Detweiler,
Alex Espinosa,
Wesley Wu,
Joshua Batson,
David Dynerman,
CLIAHUB Consortium,
Debra A Wadford,
Andreas Puschnik,
Norma F. Neff,
Vida Ahyong,
Steve Miller,
Patrick Ayscue,
Cristina M Tato,
Simon Paul,
Amy Kistler,
Joseph L. Derisi,
Emily Crawford
Posted 26 Aug 2020
bioRxiv DOI: 10.1101/2020.08.25.265074
We identify a mutation in the N gene of SARS-CoV-2 that adversely affects annealing of a commonly used RT-PCR primer; epidemiologic evidence suggests the virus retains pathogenicity and competence for spread. This reinforces the importance of using multiple targets for robust SARS-CoV-2 detection. ### Competing Interest Statement The authors have declared no competing interest.
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