Rxivist logo

Influenza-induced activation of recruited alveolar macrophages during the early inflammatory phase drives lung injury and lethality

By Clarissa M Koch, Kishore Anakella, Jennifer Yuan-Shi Hu, Mark Ciesielski, Gaurav Gadhvi, Sam Chen, Maggie Turner, Yuan Cheng, Bria M Coates, Hiam Abdala-Valencia, Paul A. Reyfman, Alexander V. Misharin, GR Scott Budinger, Deborah R. Winter, Karen M. Ridge

Posted 09 Jun 2020
bioRxiv DOI: 10.1101/2020.06.08.141309

Influenza A virus (IAV) infection significantly contributes to global mortality each year. Previous studies have shown that monocyte-derived alveolar macrophages recruited to the lung drive injury during influenza infection. However, the exact timing and mechanism of macrophage-driven lung damage is unknown. Here, we define three distinct patterns of gene expression in recruited macrophages over the first six days post-IAV infection in mice. Additionally, we demonstrate that Vim-/- mice infected with IAV exhibit decreased mortality, which was associated with a temporally altered transcriptional response in recruited macrophages. During the early inflammatory phase of IAV response, Vim-/- macrophages were characterized by a protective shift in macrophage activation. Furthermore, using a bone-marrow chimera mouse model, we demonstrate that vimentin deficiency in recruited macrophages is sufficient to confer protection from IAV-induced mortality. Taken together, our findings provide new insight into the temporal dynamics of macrophage function in response to IAV infection. ### Competing Interest Statement The authors have declared no competing interest.

Download data

  • Downloaded 174 times
  • Download rankings, all-time:
    • Site-wide: 83,389 out of 101,163
    • In immunology: 2,485 out of 3,151
  • Year to date:
    • Site-wide: 35,175 out of 101,163
  • Since beginning of last month:
    • Site-wide: 28,287 out of 101,163

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


  • 20 Oct 2020: Support for sorting preprints using Twitter activity has been removed, at least temporarily, until a new source of social media activity data becomes available.
  • 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
  • 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
  • 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
  • 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
  • 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
  • 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
  • 22 Jan 2019: Nature just published an article about Rxivist and our data.
  • 13 Jan 2019: The Rxivist preprint is live!