Rxivist logo

Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine

By Joanne Durgan, Alf H Lystad, Katherine Sloan, Sven R Carlsson, Michael I Wilson, Elena Marcassa, Rachel Ulferts, Judith Webster, Andrea F Lopez-Clavijo, Michael J Wakelam, Rupert Beale, Anne Simonsen, David Oxley, Oliver Florey

Posted 15 May 2020
bioRxiv DOI: 10.1101/2020.05.14.096115

Autophagy is a fundamental catabolic process essential for development, homeostasis and proper immune function. During autophagy, a cascade of ATG proteins target intracellular cargoes for lysosomal degradation and recycling. This pathway utilises a unique post-translational modification, the conjugation of ATG8 proteins to phosphatidylethanolamine (PE) at autophagosomes, which modulates cargo selection and maturation. ATG8 lipidation also occurs during non-canonical autophagy, a parallel pathway involving Single Membrane ATG8 Conjugation (SMAC) to endolysosomal compartments, which plays a key role in phagocytosis and other processes. It has been widely assumed that SMAC involves the same lipidation of ATG8 to PE, but this has yet to be formally tested. Here, we show that ATG8 undergoes alternative lipidation to phosphatidylserine (PS) during non-canonical autophagy/SMAC. Using mass spectrometry, we find that activation of SMAC, by pharmacological agents or during non-canonical autophagy processes such as LC3-associated phagocytosis and Influenza A virus infection, induces the covalent conjugation of ATG8 to PS, as well as PE. This alternative lipidation event is dependent on the ATG16L1 WD40 domain, and occurs at PS enriched endolysosomal membranes. Importantly, we find that the ATG8-PS and ATG8-PE adducts are differentially delipidated by isoforms of the ATG4 family, indicating significant molecular distinctions and mechanisms between these two species. Together, these results provide an important new insight into autophagy signalling, revealing an alternative form of the hallmark ATG8-lipidation event, so widely used to define and assay autophagy. Furthermore, ATG8-PS lipidation provides a specific ″molecular signature″ for non-canonical autophagy, uncovering a novel means of detecting and monitoring this emerging pathway. ### Competing Interest Statement The authors have declared no competing interest.

Download data

  • Downloaded 676 times
  • Download rankings, all-time:
    • Site-wide: 25,294 out of 103,764
    • In cell biology: 1,152 out of 5,364
  • Year to date:
    • Site-wide: 5,231 out of 103,764
  • Since beginning of last month:
    • Site-wide: 12,412 out of 103,764

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News