Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability
Philip J.M. Brouwer,
Tom G. Caniels,
Karlijn van der Straten,
Jonne L. Snitselaar,
Jonathan L. Torres,
Nisreen M.A. Okba,
Arthur E.H. Bentlage,
Marlies M. van Haaren,
Judith A. Burger,
Edith E. Schermer,
Kirsten D. Verheul,
Niels van der Velde,
Alex van der Kooi,
Jelle van Schooten,
Mariëlle J. van Breemen,
Tom P. L. Bijl,
Neeltje A. Kootstra,
W. Joost Wiersinga,
Bart L. Haagmans,
Andrew B. Ward,
Godelieve J. de Bree,
Rogier W. Sanders,
Marit J. van Gils
Posted 12 May 2020
bioRxiv DOI: 10.1101/2020.05.12.088716 (published DOI: 10.1126/science.abc5902)
Posted 12 May 2020
The rapid spread of SARS-CoV-2 has a significant impact on global health, travel and economy. Therefore, preventative and therapeutic measures are urgently needed. Here, we isolated neutralizing antibodies from convalescent COVID-19 patients using a SARS-CoV-2 stabilized prefusion spike protein. Several of these antibodies were able to potently inhibit live SARS-CoV-2 infection at concentrations as low as 0.007 μg/mL, making them the most potent human SARS-CoV-2 antibodies described to date. Mapping studies revealed that the SARS-CoV-2 spike protein contained multiple distinct antigenic sites, including several receptor-binding domain (RBD) epitopes as well as previously undefined non-RBD epitopes. In addition to providing guidance for vaccine design, these mAbs are promising candidates for treatment and prevention of COVID-19. ### Competing Interest Statement Amsterdam UMC has filed a patent application on COVID-19 monoclonal antibodies.
- Downloaded 3,680 times
- Download rankings, all-time:
- Site-wide: 1,547 out of 103,808
- In microbiology: 167 out of 9,238
- Year to date:
- Site-wide: 424 out of 103,808
- Since beginning of last month:
- Site-wide: 5,425 out of 103,808
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!