Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 62,945 bioRxiv papers from 279,208 authors.

Vibrio natriegens, a new genomic powerhouse

By Henry H Lee, Nili Ostrov, Brandon G Wong, Michaela A Gold, Ahmad S. Khalil, George M Church

Posted 12 Jun 2016
bioRxiv DOI: 10.1101/058487 (published DOI: 10.1038/s41564-019-0423-8)

Recombinant DNA technology has revolutionized biomedical research with continual innovations advancing the speed and throughput of molecular biology. Nearly all these tools, however, are reliant on Escherichia coli as a host organism, and its lengthy growth rate increasingly dominates experimental time. Here we report the development of Vibrio natriegens, a free-living bacteria with the fastest generation time known, into a genetically tractable host organism. We systematically characterize its growth properties to establish basic laboratory culturing conditions. We provide the first complete Vibrio natriegens genome, consisting of two chromosomes of 3,248,023 bp and 1,927,310 bp that together encode 4,578 open reading frames. We reveal genetic tools and techniques for working with Vibrio natriegens. These foundational resources will usher in an era of advanced genomics to accelerate biological, biotechnological, and medical discoveries.

Download data

  • Downloaded 15,138 times
  • Download rankings, all-time:
    • Site-wide: 44 out of 62,945
    • In genomics: 12 out of 4,316
  • Year to date:
    • Site-wide: 314 out of 62,945
  • Since beginning of last month:
    • Site-wide: 286 out of 62,945

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)