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Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1

By Daniel Lüdke, Charlotte Roth, Sieglinde A. Kamrad, Jana Messerschmidt, Denise Hartken, Jonas Appel, Bojan F. Hörnich, Qiqi Yan, Stefan Kusch, Melanie Klenke, Annette Gunkel, Lennart Wirthmueller, Marcel Wiermer

Posted 10 May 2020
bioRxiv DOI: 10.1101/2020.05.09.084020

IMPORTIN-α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin-α isoforms in Arabidopsis that recruit nuclear localization signal (NLS)-containing cargo proteins to the nuclear import machinery. IMP-α3/MOS6 is required genetically for full autoimmunity of the nucleotide-binding leucine-rich repeat ( NLR ) immune receptor mutant snc1 ( suppressor of npr1-1, constitutive 1 ) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin-α genes to both types of immune responses, and we analyzed potential interactions of all importin-α isoforms with SNC1. By using reverse-genetic analyses in Arabidopsis and protein-protein interaction assays in N. benthamiana we provide evidence that among the nine α-importins in Arabidopsis , IMP-α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP-α3/MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis . Significance Statement : Specific requirement for the Arabidopsis α-importin MOS6 in snc1 -mediated autoimmunity is explained by selective formation of MOS6-SNC1 nuclear import complexes. ### Competing Interest Statement The authors have declared no competing interest.

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