Functional requirement of the Arabidopsis importin-α nuclear transport receptor family in autoimmunity mediated by the NLR protein SNC1
Sieglinde A. Kamrad,
Bojan F. Hörnich,
Posted 10 May 2020
bioRxiv DOI: 10.1101/2020.05.09.084020
Posted 10 May 2020
IMPORTIN-α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin-α isoforms in Arabidopsis that recruit nuclear localization signal (NLS)-containing cargo proteins to the nuclear import machinery. IMP-α3/MOS6 is required genetically for full autoimmunity of the nucleotide-binding leucine-rich repeat ( NLR ) immune receptor mutant snc1 ( suppressor of npr1-1, constitutive 1 ) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin-α genes to both types of immune responses, and we analyzed potential interactions of all importin-α isoforms with SNC1. By using reverse-genetic analyses in Arabidopsis and protein-protein interaction assays in N. benthamiana we provide evidence that among the nine α-importins in Arabidopsis , IMP-α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP-α3/MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis . Significance Statement : Specific requirement for the Arabidopsis α-importin MOS6 in snc1 -mediated autoimmunity is explained by selective formation of MOS6-SNC1 nuclear import complexes. ### Competing Interest Statement The authors have declared no competing interest.
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