A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Philip M. Borden,
Amol V. Shivange,
Jonathan S. Marvin,
Mark A Lobas,
Paula K Zhu,
W. Sharon Zheng,
Andrew G. Yee,
Christopher P Ford,
Douglas C Rees,
Baljit S. Khakh,
Jeremy S. Dittman,
Joseph F. Cheer,
Henry A. Lester,
J. Julius Zhu,
Loren L Looger
Posted 08 Feb 2020
bioRxiv DOI: 10.1101/2020.02.07.939504
Posted 08 Feb 2020
Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein. iAChSnFR shows large fluorescence changes, rapid rise and decay kinetics, and insensitivity to most cholinergic drugs. iAChSnFR revealed large transients in a variety of slice and in vivo preparations in mouse, fish, fly and worm. iAChSnFR will be useful for the study of acetylcholine in all organisms.
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