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Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936

By Jamie Toombs, Lindsay Panther, Loren Ornelas, Chunyan Liu, Emilda Gomez, Raquel Martín-Ibáñez, Simon R Cox, Stuart J. Ritchie, Sarah E. Harris, Adele Taylor, Paul Redmond, Tom C. Russ, Lee Murphy, James D Cooper, Karen Burr, Bhuvaneish T. Selvaraj, Cathy Browne, Clive N. Svendsen, Sally A. Cowley, Ian J Deary, Siddharthan Chandran, Tara L Spires-Jones, Dhruv Sareen

Posted 05 Feb 2020
bioRxiv DOI: 10.1101/2020.02.05.935213 (published DOI: 10.1016/j.scr.2020.101851)

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

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