TAF-ChIP: An ultra-low input approach for genome wide chromatin immunoprecipitation assay
Miguel A. Andrade-Navarro,
Posted 11 Apr 2018
bioRxiv DOI: 10.1101/299727 (published DOI: 10.26508/lsa.201900318)
Posted 11 Apr 2018
Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) is a powerful technique to study transcriptional regulation. However, the requirement of millions of cells to generate results with high signal-to-noise ratio precludes it in the study of small cell populations. Here, we present a Tagmentation-Assisted Fragmentation ChIP (TAF-ChIP) and sequencing method to generate high-quality histone profiles from low cell numbers. The data obtained from the TAF-ChIP approach is amenable to standard tools for ChIP-Seq analysis, owing to its high signal-to-noise ratio. The epigenetic profiles from TAF-ChIP approach showed high agreement with conventional ChIP-Seq datasets, thereby underlining the utility of this approach.
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