Rxivist logo

Activation of macrophages by lysophosphatidic acid through the lysophosphatidic acid receptor 1 as a novel mechanism in multiple sclerosis pathogenesis.

By Jennifer Fransson, Ana Isabel Gómez, Jesús Romero-Imbroda, Oscar Fernández, Laura Leyva, Fernando Rodriguez de Fonseca, Jerold Chun, Celine Louapre, Anne Baron Van-Evercooren, Violetta Zujovic, Guillermo Estivill-Torrús, Beatriz García-Díaz

Posted 11 Dec 2019
bioRxiv DOI: 10.1101/870980

Multiple sclerosis (MS) is a neuro-inflammatory disease for which the pathogenesis remains largely unclear. Lysophosphatidic acid (LPA) is an endogenous phospholipid that is involved in multiple immune cell functions and is dysregulated in MS. Its receptor LPA1 is expressed in macrophages and regulates their activation, which is of interest due to the role of macrophage activation in MS in both destruction and repair. In this study, we studied the viable Malaga variant of LPA1-null mutation as well as pharmaceutical inhibition of LPA1 in mice with experimental autoimmune encephalomyelitis (EAE), a model of MS. LPA1 expression was also analyzed in both wild-type EAE mice and MS patient immune cells. The effect of LPA and LPA1 on macrophage activation was studied in human monocyte-derived macrophages. We show that lack of LPA1 activity induces a milder clinical course in EAE, and that Lpar1 expression in peripheral blood mononuclear cells (PBMCs) correlates with onset of relapses and severity in wild-type EAE mice. We see the same over-expression in PBMCs from MS patients during relapse compared to progressive forms of the disease, and in monocyte-derived macrophages after exposure to pro-inflammatory stimuli. In addition, LPA induced a pro-inflammatory-like response in macrophages through LPA1, providing a plausible way in which LPA and LPA1 dysregulation can lead to the inflammation seen in MS. These data show a new mechanism of LPA signaling in the pathogenesis of MS, prompting further research into its use as a therapeutic target biomarker.

Download data

  • Downloaded 134 times
  • Download rankings, all-time:
    • Site-wide: 69,062 out of 77,600
    • In neuroscience: 12,405 out of 13,900
  • Year to date:
    • Site-wide: 25,622 out of 77,600
  • Since beginning of last month:
    • Site-wide: 31,464 out of 77,600

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News