Rxivist logo

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal sediments, from a range of archaeological contexts.

Download data

  • Downloaded 539 times
  • Download rankings, all-time:
    • Site-wide: 66,632
    • In microbiology: 4,266
  • Year to date:
    • Site-wide: 145,388
  • Since beginning of last month:
    • Site-wide: 162,393

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide