jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths
Manuel Alexander Mohr,
Jonathan S. Marvin,
Emiliano Jimenez Marquez,
John J Macklin,
Douglas S. Kim,
Allan M. Wong,
Loren L. Looger,
Eric R. Schreiter,
Posted 25 Oct 2019
bioRxiv DOI: 10.1101/816660 (published DOI: 10.1038/s41592-020-0835-7)
Posted 25 Oct 2019
State-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.
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