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jYCaMP: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths

By Manuel A. Mohr, Daniel Bushey, Abhi Aggarwal, Jonathan S. Marvin, Emiliano Jimenez Marquez, Yajie Liang, Ronak Patel, John J Macklin, Chi-Yu Lee, Douglas S. Kim, Allan M. Wong, Loren L Looger, Eric R. Schreiter, Kaspar Podgorski

Posted 25 Oct 2019
bioRxiv DOI: 10.1101/816660 (published DOI: 10.1038/s41592-020-0835-7)

State-of-the-art GFP-based calcium indicators do not undergo efficient two-photon excitation at wavelengths above 1000 nm, for which inexpensive and powerful industrial femtosecond lasers are available. Here we report jYCaMP1, a yellow variant of jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1000 nm and enables improved two-color calcium imaging with RFP-based indicators.

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