Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 62,292 bioRxiv papers from 276,522 authors.

Hackflex: low cost Illumina sequencing library construction for high sample counts

By Daniela Gaio, Joyce To, Michael Liu, Leigh Monahan, Kay Anantanawat, Aaron E Darling

Posted 23 Sep 2019
bioRxiv DOI: 10.1101/779215

We developed Hackflex, a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 11 times more libraries for high-throughput Illumina sequencing to be generated at a fixed cost. We call this new method Hackflex. Quality of library preparation was tested by constructing libraries from E. coli MG1655 genomic DNA using either Hackflex, standard Nextera Flex or a variation of standard Nextera Flex in which the bead-linked transposase is diluted prior to use. We demonstrated that Hackflex can produce high quality libraries and yields a highly uniform coverage, equivalent to the standard Nextera Flex kit. Using Hackflex, we were able to achieve a per sample reagent cost of library prep of A$8.66, which is 8.23 times lower than the Standard Nextera Flex protocol at advertised retail price. An additional simple modification to the protocol enables a further price reduction of up to 11 fold or about A$6.50/sample. This method will allow researchers to construct more libraries within a given budget, thereby yielding more data and facilitating research programs where sequencing large numbers of libraries is beneficial.

Download data

  • Downloaded 1,183 times
  • Download rankings, all-time:
    • Site-wide: 5,269 out of 62,292
    • In genomics: 903 out of 4,282
  • Year to date:
    • Site-wide: 1,049 out of 62,292
  • Since beginning of last month:
    • Site-wide: 23 out of 62,292

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)