Robustness to temperature variation is an important specification for biomolecular circuit design. While cancellation of parametric temperature dependences has been shown to improve temperature robustness of period in a synthetic oscillator design, the performance of other biomolecular circuit designs in different temperature conditions is relatively unclear. Using a combination of experimental measurements and mathematical models, we assess the temperature robustness of two biomolecular circuit motifs \---| a negative feedback loop and a feedforward loop. We find that the measured responses in both circuits can change with temperature, both in the amplitude and in the transient response. We find that, in addition to the cancellation of parametric temperature dependencies, certain parameter regimes can also facilitate temperature robustness for the negative feedback loop, although at a performance cost. We discuss these parameter regimes of operation in the context of the measured data for the negative feedback loop. These results should help develop a framework for assessing and designing temperature robustness in biomolecular circuits.
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