Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,170 bioRxiv papers from 306,408 authors.

Single Cell RNAseq Reveals A Critical Role of Chloride Channels in Airway Development

By Mu He, Bing Wu, Daniel D Le, Wenlei Ye, Adriane W Sinclair, Valeria Padovano, Yuzhang Chen, Kexin Li, Rene Sit, Michelle Tan, Michael J Caplan, Norma Neff, Yuh Nung Jan, Spyros Darmanis, Lily Y. Jan

Posted 15 Aug 2019
bioRxiv DOI: 10.1101/735969

The conducting airway forms a protective mucosal barrier and is the primary target of airway disorders. To better understand how airway developmental programs are established to support air breathing and barrier functions, we constructed a single-cell atlas of the human and mouse developing trachea. In this study, we uncover hitherto unrecognized heterogeneity of cell states with distinct differentiation programs and immune features of the developing airway. In addition, we find ubiquitous expression of CFTR and ANO1/TMEM16A chloride channels in the embryonic airway epithelium. We show that genetic inactivation of TMEM16A leads to airway defects commonly seen in cystic fibrosis patients with deficient CFTR, alters the differentiation trajectory of airway basal progenitors, and results in mucus cell hyperplasia and aberrant epithelial antimicrobial expression. Together, our study illuminates conserved developmental features of the mammalian airway and implicates chloride homeostasis as a key player in regulating mucosal barrier formation and function relevant to early onset airway diseases.

Download data

  • Downloaded 630 times
  • Download rankings, all-time:
    • Site-wide: 16,455 out of 70,177
    • In developmental biology: 397 out of 2,098
  • Year to date:
    • Site-wide: 1,768 out of 70,177
  • Since beginning of last month:
    • Site-wide: 14,825 out of 70,177

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News