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Background free tracking of single RNA in living cells using catalytically inactive CasE

By Feng Gao, Yue Sun, Feng Jiang, Xiaoyue Bai, Chunyu Han

Posted 13 May 2019
bioRxiv DOI: 10.1101/635912

RNAs have important and diverse functions. Visualizing an isolated RNA in living cells provide us essential information of its roles. By now, there are two kinds of live RNA imaging systems invented, one is the MS2 system and the other is the Cas13a system. In this study, we show that when fused with split-Fp, CasE can be engineered into a live RNA tracking tool.

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