All-optical electrophysiology reveals excitation, inhibition, and neuromodulation in cortical layer 1
Linlin Z. Fan,
Urs L. Böhm,
Kiryl D Piatkevich,
Michael E. Xie,
Anne E. Takesian,
Adam E. Cohen
Posted 23 Apr 2019
bioRxiv DOI: 10.1101/614172 (published DOI: 10.1016/j.cell.2020.01.001)
Posted 23 Apr 2019
The stability of neural dynamics arises through a tight coupling of excitatory (E) and inhibitory (I) signals. Genetically encoded voltage indicators (GEVIs) can report both spikes and subthreshold dynamics in vivo , but voltage only reveals the combined effects of E and I synaptic inputs, not their separate contributions individually. Here we combine optical recording of membrane voltage with simultaneous optogenetic manipulation to probe E and I individually in barrel cortex Layer 1 (L1) neurons in awake mice. Our studies reveal how the L1 microcircuit integrates thalamocortical excitation, lateral inhibition and top-down neuromodulatory inputs. We develop a simple computational model of the L1 microcircuit which captures the main features of our data. Together, these results suggest a model for computation in L1 interneurons consistent with their hypothesized role in attentional gating of the underlying cortex. Our results demonstrate that all-optical electrophysiology can reveal basic principles of neural circuit function in vivo . One Sentence Summary All-optical electrophysiology revealed the function in awake mice of an inhibitory microcircuit in barrel cortex Layer 1.
- Downloaded 2,169 times
- Download rankings, all-time:
- Site-wide: 3,303 out of 92,253
- In neuroscience: 460 out of 16,420
- Year to date:
- Site-wide: 11,928 out of 92,253
- Since beginning of last month:
- Site-wide: 21,701 out of 92,253
Downloads over time
Distribution of downloads per paper, site-wide
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!