Rxivist logo

Differential nanoscale organisation of LFA-1 modulates T cell migration

By Michael J. Shannon, Judith Pineau, Juliette Griffié, Jesse Aaron, Tamlyn Peel, David J. Williamson, Rose Zamoyska, Andrew P. Cope, Georgina H. Cornish, Dylan M Owen

Posted 09 Apr 2019
bioRxiv DOI: 10.1101/602326 (published DOI: 10.1242/jcs.232991)

Effector T-cells rely on integrins to drive adhesion and migration to facilitate their immune function. Heterodimeric transmembrane integrin LFA-1 (αLβ2) regulates adhesion and migration through linkage of the extracellular matrix with the intracellular actin treadmill machinery. We quantitated the velocity and direction of F-actin flow in migrating T-cells alongside single molecule localisation of transmembrane and intracellular LFA-1. Our results show that retrograde actin flow positively correlated and immobile actin negatively correlated with T-cell velocity. Plasma membrane localised LFA-1 forms unique nano-clustering patterns in the leading edge, compared to the mid-focal zone, in migrating T-cells. Deleting the cytosolic phosphatase PTPN22, a negative regulator of integrin signaling, increased T-cell velocity, and leading-edge cluster co-localisation of pY397 FAK, pY416 Src family kinases and LFA-1. These data suggest that differential nanoclustering patterns of LFA-1 in migrating T-cells can instruct intracellular signalling linked with the actin treadmill. Our data presents a paradigm where T cells modulate the nanoscale organisation of adhesion and signalling molecules to fine tune their migration speed. This has implications for the regulation of immune and inflammatory responses.

Download data

  • Downloaded 459 times
  • Download rankings, all-time:
    • Site-wide: 54,432
    • In immunology: 1,470
  • Year to date:
    • Site-wide: 110,764
  • Since beginning of last month:
    • Site-wide: 110,764

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)