Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,655 bioRxiv papers from 298,484 authors.

The KZFP/KAP1 system controls transposable elements-embedded regulatory sequences in adult T cells

By Flavia Marzetta, Laia Simó-Riudalbas, Julien Duc, Evarist Planet, Sonia Verp, Priscilla Turelli, Didier Trono

Posted 19 Jan 2019
bioRxiv DOI: 10.1101/523597

Transposable elements-embedded regulatory sequences (TEeRS) are subjected to early embryonic repression through sequence-specific recruitment of KRAB zinc finger proteins (KZFPs), their cofactor KAP1/TRIM28 and associated chromatin modifiers. This modulates the TEeRS-mediated regulation of gene expression in embryonic stem cells (ESCs) and leads to DNA methylation-induced silencing. However, KZFPs are broadly expressed in adult tissues, suggesting that they control TEeRS throughout life. Confirming this hypothesis, we reveal here that the KZFP/KAP1 system exerts a highly dynamic control of TEeRS in adult human CD4+ T lymphocytes. First, we observed that in these cells many TEs are still bound by KAP1, the recruitment of which is dynamically regulated upon T cell receptor stimulation. Second, we found that KAP1 depletion induces broad transcriptional alterations in T cells, with de-repression of TE-based regulatory elements leading to the illegitimate activation of nearby genes. Finally, we show that the tissue-restricted expression of KZFPs correlates with KAP1-mediated lineage-specific chromatin signatures and transcriptional repression. These data support a model where TE-targeting KZFPs and KAP1 are important regulators of gene expression in adult human cells.

Download data

  • Downloaded 628 times
  • Download rankings, all-time:
    • Site-wide: 15,802 out of 67,655
    • In genomics: 2,009 out of 4,583
  • Year to date:
    • Site-wide: 4,515 out of 67,655
  • Since beginning of last month:
    • Site-wide: 5,545 out of 67,655

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide

Sign up for the Rxivist weekly newsletter! (Click here for more details.)