Rxivist logo

A circuit of protein-protein regulatory interactions enables polarity establishment in a bacterium

By Wei Zhao, Samuel W. Duvall, Kimberly A. Kowallis, Dylan T. Tomares, Haley N. Petitjean, W. Seth Childers

Posted 21 Dec 2018
bioRxiv DOI: 10.1101/503250

Asymmetric cell division generates specialized daughter cells that play a variety of roles including tissue morphogenesis in eukaryotes and pathogenesis in bacteria. In the gram-negative bacterium Caulobacter crescentus, asymmetric localization of two biochemically distinct signaling hubs at opposite cell poles provides the foundation for asymmetric cell division. Through a set of genetic, synthetic biology and biochemical approaches we have characterized the regulatory interactions between three scaffolding proteins. These studies have revealed that the scaffold protein PodJ functions as a central mediator for organizing the new cell signaling hub, including promoting bipolarization of the central developmental scaffold protein PopZ. In addition, we identified that the old pole scaffold SpmX serves as a negative regulator of PodJ subcellular accumulation. These two scaffold-scaffold regulatory interactions serve as the core of an integrated cell polarization circuit that is layered on top of the cell-cycle circuitry to coordinate cell differentiation and asymmetric cell division.

Download data

  • Downloaded 387 times
  • Download rankings, all-time:
    • Site-wide: 41,501 out of 88,562
    • In microbiology: 2,886 out of 7,608
  • Year to date:
    • Site-wide: 40,758 out of 88,562
  • Since beginning of last month:
    • Site-wide: 41,463 out of 88,562

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)