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Differential dominance of an allele of the Drosophila tßh gene challenges standard genetic techniques

By Christine Damrau, Anders Eriksson, Julien Colomb, Bjoern Brembs

Posted 21 Dec 2018
bioRxiv DOI: 10.1101/504332

The biogenic amine octopamine (OA) and its precursor tyramine (TA) are involved in controlling a plethora of different physiological and behavioral processes. The tyramine-ß-hydroxylase (tßh) gene encodes the enzyme catalyzing the last synthesis step from TA to OA. Here, we report differential dominance (from recessive to overdominant) of the putative null tßh nM18 allele in two behavioral measures in Buridan’s paradigm (walking speed and stripe deviation) and a proboscis extension assay in the fruit fly Drosophila melanogaster . The behavioral analysis of transgenic tßh expression experiments in mutant and wild type flies as well as of OA- and TA-receptor mutants revealed a complex interaction of both aminergic systems. Our analysis suggests that the different neuronal networks responsible for the three phenotypes show different sensitivity to tßh gene dosage. This conclusion entails important implications for standard transgenic techniques, commonly used in functional genetics.

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