Rxivist logo

Low-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware

By Tomas Aidukas, Regina Eckert, Andrew R Harvey, Laura Waller, Pavan C. Konda

Posted 02 Nov 2018
bioRxiv DOI: 10.1101/460055 (published DOI: 10.1038/s41598-019-43845-9)

The revolution in low-cost consumer photography and computation provides fertile opportunity for a disruptive reduction in the cost of biomedical imaging. Conventional approaches to low-cost microscopy are fundamentally restricted, however, to modest field of view (FOV) and/or resolution. We report a low-cost microscopy technique, implemented with a Raspberry Pi single-board computer and color camera combined with Fourier ptychography (FP), to computationally construct 25-megapixel images with sub-micron resolution. New image-construction techniques were developed to enable the use of the low-cost Bayer color sensor, to compensate for the highly aberrated re-used camera lens and to compensate for misalignments associated with the 3D-printed microscope structure. This high ratio of performance to cost is of particular interest to high-throughput microscopy applications, ranging from drug discovery and digital pathology to health screening in low-income countries. 3D models and assembly instructions of our microscope are made available for open source use.

Download data

  • Downloaded 2,742 times
  • Download rankings, all-time:
    • Site-wide: 3,800 out of 119,234
    • In pathology: 25 out of 702
  • Year to date:
    • Site-wide: 6,679 out of 119,234
  • Since beginning of last month:
    • Site-wide: 3,829 out of 119,234

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)