Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 73,401 bioRxiv papers from 319,500 authors.

BRICseq bridges brain-wide interregional connectivity to neural activity and gene expression in single animals

By Longwen Huang, Justus M. Kebschull, Daniel Furth, Simon Musall, Matthew T. Kaufman, Anne K. Churchland, Anthony M Zador

Posted 20 Sep 2018
bioRxiv DOI: 10.1101/422477

Comprehensive analysis of neuronal networks requires brain-wide measurement of connectivity, activity, and gene expression. Although high-throughput methods are available for mapping brain-wide activity and transcriptomes, comparable methods for mapping region-to-region connectivity remain slow and expensive because they require averaging across hundreds of brains. Here we describe BRICseq, which leverages DNA barcoding and sequencing to map connectivity from single individuals in a few weeks and at low cost. Applying BRICseq to the mouse neocortex, we find that region-to-region connectivity provides a simple bridge relating transcriptome to activity: The spatial expression patterns of a few genes predict region-to-region connectivity, and connectivity predicts activity correlations. We also exploited BRICseq to map the mutant BTBR mouse brain, which lacks a corpus callosum, and recapitulated its known connectopathies. BRICseq allows individual laboratories to compare how age, sex, environment, genetics and species affect neuronal wiring, and to integrate these with functional activity and gene expression.

Download data

  • Downloaded 1,970 times
  • Download rankings, all-time:
    • Site-wide: 2,773 out of 73,401
    • In neuroscience: 416 out of 13,200
  • Year to date:
    • Site-wide: 12,466 out of 73,401
  • Since beginning of last month:
    • Site-wide: 12,466 out of 73,401

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News