Rxivist logo

Dynamical Scaling And Phase Coexistence In Topologically-Constrained DNA Melting

By Y. A. G. Fosado, Davide Michieletto, D. Marenduzzo

Posted 29 Mar 2017
bioRxiv DOI: 10.1101/121673 (published DOI: 10.1103/PhysRevLett.119.118002)

There is a long-standing experimental observation that the melting of topologically constrained DNA, such as circular-closed plasmids, is less abrupt than that of linear molecules. This finding points to an important role of topology in the physics of DNA denaturation, which is however poorly understood. Here, we shed light on this issue by combining large-scale Brownian Dynamics simulations with an analytically solvable phenomenological Landau mean field theory. We find that the competition between melting and supercoiling leads to phase coexistence of denatured and intact phases at the single molecule level. This coexistence occurs in a wide temperature range, thereby accounting for the broadening of the transition. Finally, our simulations show an intriguing topology-dependent scaling law governing the growth of denaturation bubbles in supercoiled plasmids, which can be understood within the proposed mean field theory.

Download data

  • Downloaded 260 times
  • Download rankings, all-time:
    • Site-wide: 88,346
    • In biophysics: 3,411
  • Year to date:
    • Site-wide: 107,545
  • Since beginning of last month:
    • Site-wide: 107,545

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)