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A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein
Ahmed S. Abdelfattah,
Rosana S. Molina,
Matthew D. Wiens,
Thomas E. Hughes,
Douglas S. Kim,
Eric R Schreiter,
Robert E. Campbell
Posted 02 Nov 2017
bioRxiv DOI: 10.1101/213082 (published DOI: 10.1186/s12915-018-0480-0)
Posted 02 Nov 2017
Genetically-encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs enable multicolor visualization with blue or cyan-excitable fluorophores and combined use with blue or cyan-excitable optogenetic actuators. Here we report the development, structure, and validation of a new red fluorescent Ca2+ indicator, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor. We characterized the performance of K-GECO1 in cultured HeLa cells, dissociated neurons, stem cell derived cardiomyocytes, organotypic brain slices, zebrafish spinal cord in vivo, and mouse brain in vivo.
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