Rxivist logo

iSUMO - integrative prediction of functionally relevant SUMOylation events

By Xiaotong Yao, Shuvadeep Maity, Shashank Gandhi, Marcin Imielenski, Christine Vogel

Posted 01 Jun 2016
bioRxiv DOI: 10.1101/056564

Post-translational modifications by the Small Ubiquitin-like Modifier (SUMO) are essential for diverse cellular functions. Large-scale experiment and sequence-based predictions have identified thousands of SUMOylated proteins. However, the overlap between the datasets is small, suggesting many false positives with low functional relevance. Therefore, we integrated ~800 sequence features and protein characteristics such as cellular function and protein-protein interactions in a machine learning approach to score likely functional SUMOylation events (iSUMO). iSUMO is trained on a total of 24 large-scale datasets, and it predicts 2,291 and 706 SUMO targets in human and yeast, respectively. These estimates are five times higher than what existing sequence-based tools predict at the same 5% false positive rate. Protein-protein and protein-nucleic acid interactions are highly predictive of protein SUMOylation, supporting a role of the modification in protein complex formation. We note the marked prevalence of SUMOylation amongst RNA-binding proteins. We validdate iSUMO predictions by experimental or other evidence. iSUMO therefore represents a comprehensive tool to identify high-confidence, functional SUMOylation events for human and yeast.

Download data

  • Downloaded 770 times
  • Download rankings, all-time:
    • Site-wide: 15,900 out of 83,758
    • In systems biology: 448 out of 2,221
  • Year to date:
    • Site-wide: 51,455 out of 83,758
  • Since beginning of last month:
    • Site-wide: 58,189 out of 83,758

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News