Rxivist logo

An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics

By Ilias Angelidis, Lukas M. Simon, Isis E Fernandez, Maximilian Strunz, Christoph H Mayr, Flavia R Greiffo, George Tsitsiridis, Elisabeth Graf, Tim-Matthias Strom, Oliver Eickelberg, Matthias Mann, Fabian J. Theis, Herbert B Schiller

Posted 20 Jun 2018
bioRxiv DOI: 10.1101/351353 (published DOI: 10.1038/s41467-019-08831-9)

Aging promotes lung function decline and susceptibility to chronic lung diseases, which are the third leading cause of death worldwide. We used single cell transcriptomics and mass spectrometry to quantify changes in cellular activity states of 30 cell types and the tissue proteome from lungs of young and old mice. Aging led to increased transcriptional noise, indicating deregulated epigenetic control. We observed highly distinct effects of aging on cell type level, uncovering increased cholesterol biosynthesis in type-2 pneumocytes and lipofibroblasts as a novel hallmark of lung aging. Proteomic profiling revealed extracellular matrix remodeling in old mice, including increased collagen IV and XVI and decreased Fraser syndrome complex proteins and Collagen XIV. Computational integration of the aging proteome and single cell transcriptomes predicted the cellular source of regulated proteins and created a first unbiased reference of the aging lung. The lung aging atlas can be accessed via an interactive user-friendly webtool at: https://theislab.github.io/LungAgingAtlas

Download data

  • Downloaded 2,798 times
  • Download rankings, all-time:
    • Site-wide: 3,701 out of 119,118
    • In systems biology: 65 out of 2,652
  • Year to date:
    • Site-wide: 14,314 out of 119,118
  • Since beginning of last month:
    • Site-wide: 18,492 out of 119,118

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News