Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 65,568 bioRxiv papers from 290,397 authors.
Highly-efficient Cas9-mediated transcriptional programming
Benjamin W Pruitt,
Christopher D Guzman,
Daniel J Wiegand,
Jonathan L Braff,
James J Collins,
George M Church
Posted 20 Dec 2014
bioRxiv DOI: 10.1101/012880 (published DOI: 10.1038/nmeth.3312)
Posted 20 Dec 2014
The RNA-guided bacterial nuclease Cas9 can be reengineered as a programmable transcription factor by a series of changes to the Cas9 protein in addition to the fusion of a transcriptional activation domain (AD). However, the modest levels of gene activation achieved by current Cas9 activators have limited their potential applications. Here we describe the development of an improved transcriptional regulator through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to Cas9. We demonstrate its utility in activating expression of endogenous coding and non-coding genes, targeting several genes simultaneously and stimulating neuronal differentiation of induced pluripotent stem cells (iPSCs).
- Downloaded 5,558 times
- Download rankings, all-time:
- Site-wide: 358 out of 65,568
- In synthetic biology: 8 out of 620
- Year to date:
- Site-wide: 16,197 out of 65,568
- Since beginning of last month:
- Site-wide: 20,134 out of 65,568
Downloads over time
Distribution of downloads per paper, site-wide
- Top preprints of 2018
- Paper search
- Author leaderboards
- Overall metrics
- The API
- Email newsletter
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!