STRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array
By
Hannah Hochgerner,
Peter Lönnerberg,
Rebecca Hodge,
Jaromir Mikes,
Abeer Heskol,
Hermann Hubschle,
Philip Lin,
Simone Picelli,
Gioele La Manno,
Michael Ratz,
Jude Dunne,
Syed Husain,
Ed Lein,
Maithreyan Srinivasan,
Amit Zeisel,
Sten Linnarsson
Posted 20 Apr 2017
bioRxiv DOI: 10.1101/126268
(published DOI: 10.1038/s41598-017-16546-4)
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but currently no high-throughput platform has been used successfully on archived human brain samples. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost. We applied the platform to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the performance of a previous lower-throughput platform.
Download data
- Downloaded 1,701 times
- Download rankings, all-time:
- Site-wide: 9,512
- In molecular biology: 249
- Year to date:
- Site-wide: 38,193
- Since beginning of last month:
- Site-wide: 107,204
Altmetric data
Downloads over time
Distribution of downloads per paper, site-wide
PanLingua
News
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!