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Beyond the ribosome: proteome-wide secretability studies using SECRiFY

By Morgane Boone, Pathmanaban Ramasamy, D. Maddelein, D. Turan, Elien Vandermarliere, Wim Vranken, N. Callewaert

Posted 14 Jan 2018
bioRxiv DOI: 10.1101/241349

While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis up to the ribosome-associated stages are now widely available, we still lack global approaches to assay post-ribosomal processes, in particular those occurring in the eukaryotic secretory system. We developed SECRiFY to simultaneously assess the secretability of >105 heterologous protein fragments by two yeast species, S. cerevisiae and P. pastoris, using custom fragment libraries, surface display and a sequencing-based readout. SECRiFY generates datasets that enable datamining into protein features underlying secretability, and it will enable studies of the impact of secretory system perturbation on the secretable proteome.

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