Rxivist logo

Beyond the ribosome: proteome-wide secretability studies using SECRiFY

By Morgane Boone, Pathmanaban Ramasamy, D. Maddelein, D. Turan, Elien Vandermarliere, Wim Vranken, N. Callewaert

Posted 14 Jan 2018
bioRxiv DOI: 10.1101/241349

While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis up to the ribosome-associated stages are now widely available, we still lack global approaches to assay post-ribosomal processes, in particular those occurring in the eukaryotic secretory system. We developed SECRiFY to simultaneously assess the secretability of >105 heterologous protein fragments by two yeast species, S. cerevisiae and P. pastoris, using custom fragment libraries, surface display and a sequencing-based readout. SECRiFY generates datasets that enable datamining into protein features underlying secretability, and it will enable studies of the impact of secretory system perturbation on the secretable proteome.

Download data

  • Downloaded 392 times
  • Download rankings, all-time:
    • Site-wide: 49,613 out of 103,808
    • In molecular biology: 1,624 out of 3,584
  • Year to date:
    • Site-wide: 70,138 out of 103,808
  • Since beginning of last month:
    • Site-wide: 45,908 out of 103,808

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)