Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 67,594 bioRxiv papers from 298,144 authors.

First nuclear genome assembly of an extinct moa species, the little bush moa (Anomalopteryx didiformis)

By Alison Cloutier, Timothy B. Sackton, Phil Grayson, Scott V. Edwards, Allan J. Baker

Posted 09 Feb 2018
bioRxiv DOI: 10.1101/262816

High throughput sequencing (HTS) has revolutionized the field of ancient DNA (aDNA) by facilitating recovery of nuclear DNA for greater inference of evolutionary processes in extinct species than is possible from mitochondrial DNA alone. We used HTS to obtain ancient DNA from the little bush moa (Anomalopteryx didiformis), one of the iconic species of large, flightless birds that became extinct following human settlement of New Zealand in the 13th century. In addition to a complete mitochondrial genome at 249.9X depth of coverage, we recover almost 900 Mb of the moa nuclear genome by mapping reads to a high quality reference genome for the emu (Dromaius novaehollandiae). This first nuclear genome assembly for moa covers approximately 75% of the 1.2 Gb emu reference with sequence contiguity sufficient to identify more than 85% of bird universal single-copy orthologs. From this assembly, we isolate 40 polymorphic microsatellites to serve as a community resource for future population-level studies in moa. We also compile data for a suite of candidate genes associated with vertebrate limb development and show that the wingless moa phenotype is likely not attributable to gene loss or pseudogenization among this candidate set. We also identify potential function-altering coding sequence variants in moa for future experimental assays.

Download data

  • Downloaded 1,623 times
  • Download rankings, all-time:
    • Site-wide: 3,486 out of 67,590
    • In evolutionary biology: 165 out of 4,484
  • Year to date:
    • Site-wide: 9,400 out of 67,590
  • Since beginning of last month:
    • Site-wide: 27,169 out of 67,590

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News