Rxivist logo

Transcription Activation Of Early Human Development Suggests DUX4 As An Embryonic Regulator

By Virpi Töhönen, Shintaro Katayama, Liselotte Vesterlund, Mona Sheikhi, Liselotte Antonsson, Giuditta Filippini-Cattaneo, Marisa Jaconi, Anna Johnsson, Sten Linnarsson, Outi Hovatta, Juha Kere

Posted 02 Apr 2017
bioRxiv DOI: 10.1101/123208

In order to better understand human preimplantation development we applied massively parallel RNA sequencing on 337 single cells from oocytes up to 8-cell embryo blastomeres. Surprisingly, already before zygote pronuclear fusion we observed drastic changes in the transcriptome compared to the unfertilized egg: 1,804 gene transcripts and 32 repeat elements become more abundant, among these the double-homeobox gene DUX4. Several genes previously identified as DUX4 targets, such as CCNA1, KHDC1L and ZSCAN4, as well as several members of the RFPLs, TRIMS and PRAMEFs1 were accumulated in 4-cell stage blastomeres, suggesting DUX4 as an early regulator. In the 8-cell stage, we observed two distinct cell types — a transcript-poor cell type, and a transcript-rich cell type with many Alu repeats and accumulated markers for pluripotency and stemness, telomere elongation and growth. In summary, this unprecedented detailed view of the first three days of human embryonic development reveals more complex changes in the transcriptome than what was previously known.

Download data

  • Downloaded 1,314 times
  • Download rankings, all-time:
    • Site-wide: 13,912
    • In developmental biology: 218
  • Year to date:
    • Site-wide: 33,144
  • Since beginning of last month:
    • Site-wide: 33,528

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)