Rxivist logo

RecNW: A fast pairwise aligner for targeted sequencing

By Alexandre Yahi, Tuuli Lappalainen, Pejman Mohammadi, Nicholas P Tatonetti

Posted 19 Jul 2018
bioRxiv DOI: 10.1101/371989

Motivation: Targeted sequencing aims at in-depth analysis of specific genomic loci through high-throughput sequencing for applications such as resequencing or CRISPR gene editing. These applications require exact pairwise alignment algorithms to fully characterize large amounts of reads by comparison to the targeted locus, or reference. Optimal solutions to this alignment problem are provided by classic implementations of the global and semi-global versions of Needleman-Wunsch algorithms, but they remain computationally expensive due to their quadratic complexity in time and space. Implementation: In this paper we present RecNW, an open source C++ exact aligner packaged for Python that implements the semi-global version of the Needleman-Wunsch algorithm with affine gap penalty. RecNW utilizes low complexity of targeted sequencing libraries by aligning only unique reads, and recurrently using blocs of the alignment matrix between reads based on their similarities. Through this, RecNW performs exact alignment on average more than four times faster than gold standard comparable software.

Download data

  • Downloaded 401 times
  • Download rankings, all-time:
    • Site-wide: 53,495 out of 116,126
    • In bioinformatics: 5,616 out of 9,552
  • Year to date:
    • Site-wide: 87,360 out of 116,126
  • Since beginning of last month:
    • Site-wide: 100,767 out of 116,126

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News