High dimensional analyses of cells dissociated from cryopreserved synovial tissue
By
Laura T. Donlin,
Deepak A. Rao,
Kevin Wei,
Kamil Slowikowski,
Mandy J. McGeachy,
Jason D. Turner,
Nida Meednu,
Fumitaka Mizoguchi,
Maria Gutierrez-Arcelus,
David J. Lieb,
Joshua Keegan,
Kaylin Muskat,
Joshua Hillman,
Cristina Rozo,
Edd Ricker,
Thomas M. Eisenhaure,
Shuqiang Li,
Edward P Browne,
Adam Chicoine,
Danielle Sutherby,
Akiko Noma,
Accelerating Medicines Partnership: RA/SLE Network,
Chad Nusbaum,
Stephen Kelly,
Alessandra B. Pernis,
Lionel B. Ivashkiv,
Susan M. Goodman,
William H. Robinson,
Paul J. Utz,
James A. Lederer,
Ellen M. Gravallese,
Brendan F. Boyce,
Nir Hacohen,
Costantino Pitzalis,
Peter K Gregersen,
Gary S. Firestein,
Soumya Raychaudhuri,
Larry W. Moreland,
V. Michael Holers,
Vivian P. Bykerk,
Andrew Filer,
David L. Boyle,
Michael B. Brenner,
Jennifer H. Anolik
Posted 19 Mar 2018
bioRxiv DOI: 10.1101/284844
(published DOI: 10.1186/s13075-018-1631-y)
Background: Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership (AMP) RA/SLE network aims to deconstruct autoimmune pathology by examining cells within target tissues through multiple high-dimensional assays. Robust standardized protocols need to be developed before cellular phenotypes at a single cell level can be effectively compared across patient samples.
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