High dimensional analyses of cells dissociated from cryopreserved synovial tissue
Laura T. Donlin,
Deepak A. Rao,
Mandy J. McGeachy,
Jason D. Turner,
David J. Lieb,
Thomas M. Eisenhaure,
Edward P Browne,
Accelerating Medicines Partnership: RA/SLE Network,
Alessandra B. Pernis,
Lionel B. Ivashkiv,
Susan M. Goodman,
William H. Robinson,
Paul J. Utz,
James A. Lederer,
Ellen M. Gravallese,
Brendan F. Boyce,
Peter K Gregersen,
Gary S. Firestein,
Larry W. Moreland,
V. Michael Holers,
Vivian P. Bykerk,
David L. Boyle,
Michael B. Brenner,
Jennifer H. Anolik
Posted 19 Mar 2018
bioRxiv DOI: 10.1101/284844 (published DOI: 10.1186/s13075-018-1631-y)
Posted 19 Mar 2018
Background: Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership (AMP) RA/SLE network aims to deconstruct autoimmune pathology by examining cells within target tissues through multiple high-dimensional assays. Robust standardized protocols need to be developed before cellular phenotypes at a single cell level can be effectively compared across patient samples.
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