Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,308 bioRxiv papers from 307,086 authors.

Molecular organization of CA1 interneuron classes

By Kenneth D. Harris, Lorenza Magno, Linda Katona, Peter Lönnerberg, Ana B. Muñoz Manchado, Peter Somogyi, Nicoletta Kessaris, Sten Linnarsson, Jens Hjerling Leffler

Posted 16 Dec 2015
bioRxiv DOI: 10.1101/034595

GABAergic interneurons are key regulators of hippocampal circuits, but our understanding of the diversity and classification of these cells remains controversial. Here we analyze the organization of interneurons in the CA1 area, using the combinatorial patterns of gene expression revealed by single-cell mRNA sequencing (scRNA-seq). This analysis reveals a 5-level hierarchy of cell classes. Most of the predicted classes correspond closely to known interneuron types, allowing us to predict a large number of novel molecular markers of these classes. In addition we identified a major new interneuron population localized at the border of strata radiatum and lacunosum-moleculare that we term "R2C2 cells" after their characteristic combinatorial expression of Rgs12, Reln, Cxcl14, and Cpne5. Several predictions of this classification scheme were verified using in situ hybridization and immunohistochemistry, providing further confidence in the gene expression patterns and novel classes predicted by the single cell data.

Download data

  • Downloaded 1,090 times
  • Download rankings, all-time:
    • Site-wide: 7,138 out of 70,262
    • In neuroscience: 1,138 out of 12,640
  • Year to date:
    • Site-wide: 10,880 out of 70,262
  • Since beginning of last month:
    • Site-wide: 17,209 out of 70,262

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

Sign up for the Rxivist weekly newsletter! (Click here for more details.)


News