A Complete Electron Microscopy Volume Of The Brain Of Adult Drosophila melanogaster
J. Scott Lauritzen,
Camenzind G. Robinson,
Corey B. Fisher,
Steven A. Calle-Schuler,
Iqbal J. Ali,
Eric T. Trautman,
Gregory S. X. E. Jefferis,
Richard D Fetter,
Davi D. Bock
Posted 22 May 2017
bioRxiv DOI: 10.1101/140905 (published DOI: 10.1016/j.cell.2018.06.019)
Posted 22 May 2017
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly. We validated the dataset by tracing brain-spanning circuitry involving the mushroom body (MB), intensively studied for its role in learning. Here we describe the complete set of olfactory inputs to the MB; find a new cell type providing driving input to Kenyon cells (the intrinsic MB neurons); identify neurons postsynaptic to Kenyon cell dendrites; and find that axonal arbors providing input to the MB calyx are more tightly clustered than previously indicated by light-level data. This freely available EM dataset will significantly accelerate Drosophila neuroscience.
- Downloaded 8,096 times
- Download rankings, all-time:
- Site-wide: 920
- In neuroscience: 44
- Year to date:
- Site-wide: 23,371
- Since beginning of last month:
- Site-wide: 19,226
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!