A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality
Tanya L. Daigle,
Travis A. Hage,
Matthew T. Valley,
Rylan S. Larsen,
Marc M Takeno,
Lucas T Graybuck,
Karla E. Hirokawa,
Julie A. Harris,
Douglas R. Ollerenshaw,
Christopher A. Baker,
Jonathan T Ting,
Susan M. Sunkin,
Edward S. Boyden,
Nuno da Costa,
Posted 25 Nov 2017
bioRxiv DOI: 10.1101/224881 (published DOI: 10.1016/j.cell.2018.06.035)
Posted 25 Nov 2017
Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating the study of their function. We here report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically-defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.
- Downloaded 11,668 times
- Download rankings, all-time:
- Site-wide: 608
- In neuroscience: 25
- Year to date:
- Site-wide: 5,760
- Since beginning of last month:
- Site-wide: 5,530
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!