Rxivist logo

Nuclear RNA concentration coordinates RNA production with cell size in human cells

By Scott Berry, Micha Müller, Lucas Pelkmans

Posted 17 May 2021
bioRxiv DOI: 10.1101/2021.05.17.444432

Unlike its DNA template, RNA abundance and synthesis rates increase with cell size, as part of a mechanism of cellular RNA concentration homeostasis. Here, we study this scaling phenomenon in human cells by combining genome-wide perturbations with quantitative single-cell measurements. Despite relative ease in perturbing RNA synthesis, we find that RNA concentrations remain highly constant. Systems-level analysis indicates that perturbations that would lead to increased nuclear mRNA abundance result in downregulation of mRNA synthesis. This is associated with reduced levels of several transcription-associated proteins and protein states that are normally coordinated with RNA production in single cells, including RNA polymerase II (Pol II) itself. Acute shut-down of nuclear RNA degradation, elevation of nuclear mRNA levels, and mathematical modelling indicate that mammalian cells achieve RNA concentration homeostasis by an mRNA-based negative feedback on transcriptional activity in the nucleus. Ultimately, this acts to robustly scale Pol II abundance with cell volume and coordinate mRNA synthesis and degradation.

Download data

  • Downloaded 2,506 times
  • Download rankings, all-time:
    • Site-wide: 8,263
    • In molecular biology: 191
  • Year to date:
    • Site-wide: 8,214
  • Since beginning of last month:
    • Site-wide: 13,174

Altmetric data


Downloads over time

Distribution of downloads per paper, site-wide


PanLingua

News