Stability, affinity and chromatic variants of the glutamate sensor iGluSnFR
Jonathan S. Marvin,
Daniel E. Wilson,
Johannes Alexander Müeller,
Francisco José Urra Quiroz,
Justin P Little,
Ariana N. Tkachuk,
Adam W Hantman,
Samuel S.-H. Wang,
Edwin R Chapman,
David A. DiGregorio,
Loren L. Looger
Posted 15 Dec 2017
bioRxiv DOI: 10.1101/235176 (published DOI: 10.1038/s41592-018-0171-3)
Posted 15 Dec 2017
Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of the glutamate sensor iGluSnFR that are functionally brighter; can detect sub-micromolar to millimolar concentrations of glutamate; and have blue, green or yellow emission profiles. These variants allow in vivo imaging where original-iGluSnFR was too dim, reveal glutamate transients at individual spine heads, and permit kilohertz imaging with inexpensive, powerful fiber lasers.
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