Rxivist logo

Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 70,230 bioRxiv papers from 306,634 authors.

50 Hz volumetric functional imaging with continuously adjustable depth of focus

By Rongwen Lu, Masashi Tanimoto, Minoru Koyama, Na Ji

Posted 28 Dec 2017
bioRxiv DOI: 10.1101/240069 (published DOI: 10.1364/boe.9.001964)

Understanding how neural circuits control behavior requires monitoring a large population of neurons with high spatial resolution and volume rate. Here we report an axicon-based Bessel beam module with continuously adjustable depth of focus (CADoF), which turns frame rate into volume rate by extending the excitation focus in axial direction while maintaining high lateral resolutions. Cost-effective and compact, this CADoF Bessel module can be easily integrated into existing two-photon fluorescence microscopes. Simply translating one of the relay lenses along its optical axis enabled continuous adjustment of the focal length. We used this module to simultaneously monitor activity of spinal projection neurons extending over 60 micron depth in larval zebrafish at 50 Hz volume rate with adjustable imaging thickness.

Download data

  • Downloaded 832 times
  • Download rankings, all-time:
    • Site-wide: 11,013 out of 70,235
    • In neuroscience: 1,768 out of 12,633
  • Year to date:
    • Site-wide: 34,434 out of 70,235
  • Since beginning of last month:
    • Site-wide: 67,742 out of 70,235

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)