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Spatial organization of the somatosensory cortex revealed by cyclic smFISH

By Simone Codeluppi, Lars E. Borm, Amit Zeisel, Gioele La Manno, Josina A van Lunteren, Camilla I. Svensson, Sten Linnarsson

Posted 04 Mar 2018
bioRxiv DOI: 10.1101/276097

The global efforts towards the creation of a molecular census of the brain using single-cell transcriptomics is generating a large catalog of molecularly defined cell types lacking spatial information. Thus, new methods are needed to map a large number of cell-specific markers simultaneously on large tissue areas. Here, we developed a cyclic single molecule fluorescence in situ hybridization methodology and defined the cellular organization of the somatosensory cortex using markers identified by single-cell transcriptomics.

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