Rxivist logo

Rapid progression is associated with lymphoid follicle dysfunction in SIV-infected infant rhesus macaques

By Matthew P. Wood, Chloe I. Jones, Adriana Lippy, Brian G. Oliver, Brynn Walund, Katherine A. Fancher, Bridget S. Fisher, Piper J. Wright, James T. Fuller, Patience Murapa, Jakob Habib, Maud Mavigner, Ann Chahroudi, D. Noah Sather, Deborah H Fuller, Donald L. Sodora

Posted 01 Jan 2021
bioRxiv DOI: 10.1101/2020.12.31.424784

HIV-infected infants are at an increased risk of progressing rapidly to AIDS in the first weeks of life. Here, we evaluated immunological and virological parameters in 25 SIV-infected infant rhesus macaques to understand the factors influencing a rapid disease outcome. Infant macaques were infected with SIVmac251 and monitored for 10 to 17 weeks post-infection. SIV-infected infants were divided into either typical (TypP) or rapid (RP) progressor groups based on levels of plasma anti-SIV antibody levels and SIV plasma viral load (with RP infants having low SIV-specific antibodies and high viral loads). Following SIV infection, 11 out of 25 infant macaques exhibited an RP phenotype, with 5 of these succumbing to AIDS-related infections. Interestingly, the TypP and RP infants were similar in their CD4 depletion and activation of CD8 T cells as measured by the levels of HLA-DR on the cell surface. However, differences between the two groups were identified in other immune cell populations, including a failure to expand activated memory (CD21-CD27+) B cells in peripheral blood in RP infant macaques, as well as reduced levels of germinal center (GC) B cells and T follicular helper (Tfh) cells in spleens (4- and 10-weeks post-SIV). Reduced B cell proliferation in splenic germinal GCs was associated with increased SIV+ cell density and follicular type 1 interferon (IFN)-induced immune activation. Further analyses determined that at 2-weeks post SIV infection TypP infants exhibited elevated levels of the GC-inducing chemokine CXCL13 in plasma, as well as significantly lower levels of viral envelope diversity compared to RP infants. Our findings provide evidence that early viral and immunologic events following SIV infection contributes to impairment of B cells, Tfh cells and germinal center formation, ultimately impeding the development of SIV-specific antibody responses in rapidly progressing infant macaques.

Download data

  • Downloaded 40 times
  • Download rankings, all-time:
    • Site-wide: 126,798
    • In immunology: 3,635
  • Year to date:
    • Site-wide: 27,850
  • Since beginning of last month:
    • Site-wide: 27,850

Altmetric data

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)