Rxivist logo

Context-dependent effects of IL-2 rewire immunity into distinct cellular circuits

By Carly E. Whyte, Kailash Singh, Oliver T. Burton, Meryem Aloulou, Alena Moudra, Carlos P. Roca, Francisco J. Naranjo, FĂ©lix Lombard-Vadnais, Lubna Kouser, Tino Hochepied, Timotheus Y. F. Halim, Susan Schlenner, Sylvie Lesage, James Dooley, Adrian Liston

Posted 20 Dec 2020
bioRxiv DOI: 10.1101/2020.12.18.423431

Interleukin 2 (IL-2) is a key homeostatic cytokine, with potential therapeutic applications in both immunogenic and tolerogenic immune modulation. Clinical application has been hampered by pleiotropic functionality and wide-spread receptor expression, with unexpected adverse events during trials. To characterize the IL-2 homeostatic network, we developed a novel mouse strain allowing IL-2 production to be diverted. Rewiring of IL-2 production to diverse leukocyte sources allowed the identification of contextual influences over IL-2 impact. Network analysis identified a priority access for Tregs, and a competitive fitness cost induced among both Tregs and conventional CD4 T cells for IL-2 production. CD8 T cells and NK cells, by contrast, exhibited a preference for autocrine IL-2 production. IL-2 sourced from dendritic cells amplified the Treg circuit, while IL-2 produced by B cells induced two context-dependent circuits: dramatic expansion of CD8+ Tregs and ILC2 cells. The former was associated with an unexpected concentration of rare CD8+ Tregs in B cell zones, while the latter drove a downstream, IL-5-mediated, eosinophilic circuit. The source-specific effects demonstrate the contextual influence of IL-2 function and potentially explain unexpected adverse effects observed during clinical trials of exogenous IL-2. Targeted IL-2 production therefore has the potential to amplify or quench particular circuits in the IL-2 network, based on clinical desirability. Graphical abstract O_FIG_DISPLAY_L [Figure 1] M_FIG_DISPLAY C_FIG_DISPLAY

Download data

  • Downloaded 523 times
  • Download rankings, all-time:
    • Site-wide: 44,734
    • In immunology: 1,185
  • Year to date:
    • Site-wide: 1,163
  • Since beginning of last month:
    • Site-wide: 999

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide


Sign up for the Rxivist weekly newsletter! (Click here for more details.)