Rxivist combines preprints from bioRxiv with data from Twitter to help you find the papers being discussed in your field. Currently indexing 63,016 bioRxiv papers from 279,490 authors.

Analysis of entire transparent rodent bodies could provide holistic information on biological systems in health and disease. However, it has been challenging to reliably image and quantify signal from endogenously expressed fluorescent proteins in large cleared mouse bodies due to the low signal contrast. Here, we devised a pressure driven, nanobody based whole-body immunolabeling technology to enhance the signal of fluorescent proteins by up to two orders of magnitude. This allowed us to image subcellular details in transparent mouse bodies through bones and highly autofluorescent tissues, and perform quantifications. We visualized for the first-time whole-body neuronal connectivity of an entire adult mouse and discovered that brain trauma induces degeneration of peripheral axons. We also imaged meningeal lymphatic vessels and immune cells through the intact skull and vertebra in naive animals and trauma models. Thus, our new approach can provide an unbiased holistic view of biological events affecting the nervous system and the rest of the body.

Download data

• Downloaded 11,943 times
• Download rankings, all-time:
  • Site-wide: 75 out of 63,016
  • In neuroscience: 11 out of 11,260
• Year to date:
  • Site-wide: 273 out of 63,016
• Since beginning of last month:
  • Site-wide: 1,625 out of 63,016

Altmetric data

Downloads over time

Distribution of downloads per paper, site-wide