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RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis

By Shu Zhang, Nadine Uebelmesser, Natasa Josipovic, Giada Forte, Johan A. Slotman, Michael Chiang, Henrike Gothe, Eduardo Gade Gusmao, Christian Becker, Janine Altmueller, Adriaan B. Houtsmuller, Vassilis Roukos, Kerstin S. Wendt, Davide Marenduzzo, Argyris Papantonis

Posted 27 Oct 2020
bioRxiv DOI: 10.1101/2020.10.27.356915

Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces. Mitotic cell division induces drastic chromosome condensation, but following reentry into the G1 cell cycle phase, condensed chromosomes unwind to reestablish interphase organization. Here, we use a cell line allowing auxin-mediated degradation of RNA polymerase II to test its role in this transition. In situ Hi-C showed that RNAPII is required for compartment and loop formation following mitosis. RNAPs often counteract loop extrusion and, in their absence, longer and more prominent loops arise. Evidence from chromatin fractionation, super-resolution imaging and in silico modeling attribute these effects to RNAPII-mediated cohesin loading at active promoters upon reentry into G1. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture. ### Competing Interest Statement The authors have declared no competing interest.

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