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Major functional bias for mitochondrial complexes in genome-wide CRISPR screens

By Mahfuzur Rahman, Maximilian Billmann, Michael Costanzo, Michael Aregger, Amy H. Y. Tong, Katherine Chan, Henry N. Ward, Kevin R. Brown, Brenda J. Andrews, Charles Boone, Jason Moffat, Chad L. Myers

Posted 31 Aug 2020
bioRxiv DOI: 10.1101/2020.08.31.273730

We present FLEX (Functional evaluation of experimental perturbations), a pipeline that leverages several functional annotation resources to establish reference standards for benchmarking human genome-wide CRISPR screen data and methods for analyzing them. We apply FLEX to analyze data from the diverse cell line screens generated by the DepMap project. We identify a dominant mitochondria-associated signal, which our time-resolved CRISPR screens and analysis suggests may reflect screen dynamics and protein stability effects rather than genetic dependencies. ### Competing Interest Statement J.M. is a shareholder in Northern Biologics and Pionyr Immunotherapeutics, and is an advisor and shareholder of Century Therapeutics and Aelian Biotechnology.

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