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Accurate spliced alignment of long RNA sequencing reads

By Kristoffer Sahlin, Veli Mäkinen

Posted 03 Sep 2020
bioRxiv DOI: 10.1101/2020.09.02.279208

Long-read RNA sequencing techniques are quickly establishing themselves as the primary sequencing technique to study the transcriptome landscape. Many such analyses are dependent upon splice alignment of reads to the genome. However, the error rate and sequencing length of long-read technologies create new challenges for accurately aligning these reads. We present an alignment method uLTRA that, on simulated and synthetic data, shows higher accuracy over state-of-the-art with substantially higher accuracy for small exons. We show several examples on biological data where uLTRA aligns to known and novel isoforms with exon structures that are not detected with other aligners. uLTRA is available at <https://github.com/ksahlin/ultra>. ### Competing Interest Statement The authors have declared no competing interest.

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