Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore
John R Tyson,
Ji Hyun Choi,
Andrew D Smith,
Sam J Wilson,
Terrance P Snutch,
Nick J Loman,
Posted 04 Sep 2020
bioRxiv DOI: 10.1101/2020.09.04.283077
Posted 04 Sep 2020
Genome sequencing has been widely deployed to study the evolution of SARS-CoV-2 with more than 90,000 genome sequences uploaded to the GISAID database. We published a method for SARS-CoV-2 genome sequencing (<https://www.protocols.io/view/ncov-2019-sequencing-protocol-bbmuik6w>) online on January 22, 2020. This approach has rapidly become the most popular method for sequencing SARS-CoV-2 due to its simplicity and cost-effectiveness. Here we present improvements to the original protocol: i) an updated primer scheme with 22 additional primers to improve genome coverage, ii) a streamlined library preparation workflow which improves demultiplexing rate for up to 96 samples and reduces hands-on time by several hours and iii) cost savings which bring the reagent cost down to £10 per sample making it practical for individual labs to sequence thousands of SARS-CoV-2 genomes to support national and international genomic epidemiology efforts. ### Competing Interest Statement J.R.T., J.Q., and N.J.L. have all received travel expenses and accommodation from Oxford Nanopore Technologies to speak at organised events. P.J. and D.S. are employees of Oxford Nanopore Technologies.
- Downloaded 1,623 times
- Download rankings, all-time:
- Site-wide: 8,975
- In genomics: 1,028
- Year to date:
- Site-wide: 733
- Since beginning of last month:
- Site-wide: 1,507
Downloads over time
Distribution of downloads per paper, site-wide
- 27 Nov 2020: The website and API now include results pulled from medRxiv as well as bioRxiv.
- 18 Dec 2019: We're pleased to announce PanLingua, a new tool that enables you to search for machine-translated bioRxiv preprints using more than 100 different languages.
- 21 May 2019: PLOS Biology has published a community page about Rxivist.org and its design.
- 10 May 2019: The paper analyzing the Rxivist dataset has been published at eLife.
- 1 Mar 2019: We now have summary statistics about bioRxiv downloads and submissions.
- 8 Feb 2019: Data from Altmetric is now available on the Rxivist details page for every preprint. Look for the "donut" under the download metrics.
- 30 Jan 2019: preLights has featured the Rxivist preprint and written about our findings.
- 22 Jan 2019: Nature just published an article about Rxivist and our data.
- 13 Jan 2019: The Rxivist preprint is live!