Author: David A Agard

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Preprints

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

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A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing

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Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy

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A simple and robust procedure for preparing graphene-oxide cryo-EM grids

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Practical considerations for using K3 cameras in CDS mode for high-resolution and high-throughput single particle cryo-EM

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CryoEM and AI reveal a structure of SARS-CoV-2 Nsp2, a multifunctional protein involved in key host processes.

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A nucleus-like compartment shields bacteriophage DNA from CRISPR-Cas and restriction nucleases

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Atomic structure of Hsp90:Cdc37:Cdk4 reveals Hsp90 regulates kinase via dramatic unfolding

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General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM

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A molecular pore spans the double membrane of the coronavirus replication organelle

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Amino and PEG-Amino Graphene Oxide Grids Enrich and Protect Samples for High-resolution Single Particle Cryo-electron Microscopy

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Microtubules form by progressively faster tubulin accretion, not by nucleation-elongation

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Structures of the active HER2/HER3 receptor complex reveal dynamics at the dimerization interface induced by binding of a single ligand

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GR chaperone cycle mechanism revealed by cryo-EM: inactivation of GR by GR:Hsp90:Hsp70:Hop client-loading complex

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The Atomic Structure of the Microtubule-Nucleating γ-Tubulin Small Complex and its Implications for Regulation

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GR chaperone cycle mechanism revealed by cryo-EM: reactivation of GR by the GR:Hsp90:p23 client-maturation complex

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AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction

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Structural Basis of Mitochondrial Receptor Binding and GTP Driven Conformational Constriction by Dynamin-Related Protein 1

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Mind the gap: micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

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Cryo-EM structures reveal a multistep mechanism of Hsp90 activation by co-chaperone Aha1

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Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1

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CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleation

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Electron Cryo-Tomography Provides Insight into Procentriole Architecture and Assembly Mechanism

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Entropy Regularized Deconvolution of Cellular Cryo-Transmission Electron Tomograms

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Cryo-EM reveals the dynamic interplay between mitochondrial Hsp90 and SdhB folding intermediates

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Cryo-EM analysis of human mitochondrial Hsp90 in multiple tetrameric states

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Modulation of mitochondrial Hsp90 (TRAP1) ATPase activity by calcium and magnesium

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Spc110 N-Terminal Domains Act Independently to Mediate Stable γ-Tubulin Small Complex Binding and γ-Tubulin Ring Complex Assembly

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Mechanistic Origins of Dynamic Instability in Filaments from the Phage Tubulin, PhuZ

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The {varphi}PA3 Phage Nucleus is Enclosed by a Self-Assembling, 2D Crystalline Lattice

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Electron Cryo-Tomography Structure of Axonemal Doublet Microtubule from Tetrahymena thermophila

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Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 null Tetrahymena cells reveals functional MIPs

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Hsp70 chaperone blocks α-synuclein oligomer formation via a novel engagement mechanism

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Structural features of mTORC2 that control substrate-specific activities.

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Novel Small Molecules Targeting the Intrinsically Disordered Structural Ensemble of α-Synuclein Protect Against Diverse α-Synuclein Mediated Dysfunctions

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XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions

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The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles

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